Mamman et al November 2008

• | Print |

Sokoto Journal of Veterinary Sciences, 7(2):14-16

Disinfectant effects of Purit®, Z-Germicide® and Carcil® on bacterial isolates from hatcheries in Kaduna state, Nigeria

PH Mamman, HM Kazeem, JU Umoh, FB Mosimabale, CN Kwanashie and AM Wakawa

Abstract

Hatcheries in Kaduna State, Nigeria were investigated for isolation of aerobic bacteria. The following were isolated: Escherichia coli, Proteus sp, Pseudomonas sp, Staphylococcus aureus, Staphylococcus sp and Micrococcus sp. On these isolates, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and kill-time of two commonly used and one relatively new disinfectant were determined. The two commonly used disinfectants were Purit® (chlorhexidine gluconate Bp 0.3 % w/v and cetrimide Bp 3.0 % w/v) and Z-germicide® (7 % tar acid phenol and 2 % cresol). The new one was Carcil® (Alkyl-benzyl-dimethyl ammonium chloride). It was observed that Purit® was most effective against E.coli since it required the lowest concentration of 0.00026 % for 1 minute to kill the bacteria. Z-germicide® was found to be effective at 0.00063 % for 5 minutes and Carcil® required 0.0031 % for 10 seconds. Purit® was also found to be the most effective of the three disinfectants on Pseudomonas sp but Z-germicide® showed best results on Proteus sp.