Mahmuda et al. 2017

Sokoto Journal of Veterinary Sciences, Volume 15 (Number 1). April, 2017


Monoclonal antibodies in immunodiagnostic assays: a review of recent applications

A Mahmuda1,2, F Bande3, N Abdulhaleem4, KJK Al-Zihiry5, RA Majid1, RA Hamat1, WO Abdullah6 & N Zasmy1*

1.                  Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Malaysia

2.                  Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria

3.                  Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Malaysia

4.                  Department of Biology, College of Science, University of Anbar, Anbar, Iraq

5.                  Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, Iraq

6.                  Faculty of Medicine and Health Sciences, Islamic Science University of Malaysia, Kuala Lumpur, Malaysia


*Correspondence: Tel.: +60132213516; E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.



Monoclonal antibodies (mAbs) have proven to be effective biological reagents in the immunodiagnostic assays. This is due to their binding accuracy to many pathogens, thus, making them valuable research tools. Since the discovery of hybridoma technology by Kohler and Milstein, the use of monoclonal antibodies produced by the hybrid cells have been employed in diagnosis of several diseases. Monoclonal antibody production has several procedures with considerable variations, but the principles remain the same. Improvements in the field of cell culture technology have led to the production of improved qualities of monoclonal antibodies. In general, these antibodies are important biomedical reagents used in research, especially in the field of laboratory diagnostics for a number of different types of diseases in humans and animals. Some of the areas where application of monoclonal antibodies triumph are herein discussed. This review is aimed to assess various diagnostic assay techniques where monoclonal antibodies are applied in order to provide a first-hand information, especially for beginners in monoclonal antibody production, characterisation, evaluation and /or its applications in research and diagnosis.

Keywords: Monoclonal antibodies, immunodiagnostic, assays, recent applications

Online Stats

We have 6 guests and no members online

Site Stats

Web Links
Articles View Hits