Olurode & Ajala, 2016
Sokoto Journal of Veterinary Sciences (2016) 14(3)
Effects of storage temperature and extension media on motility of caprine spermatozoa
SA Olurode1* & O Ajala2
1. College of Veterinary Medicine, Federal University of Agriculture, Abeokuta, PMB 2240, Ogun state
2. Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan
*Correspondence: Tel.: +2348038607417; E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
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Abstract
The survivability of caprine spermatozoa was compared in semen extenders prepared from the conventional egg-yolk citrate and goat-milk citrate. Motility was comparable in both sets of extenders during the first 24 hours post-extension when extended ejaculates were stored at either room temperature (28 OC) or refrigerator temperature (5 OC). Motility in goat milk citrate extenders at 5OC was significantly higher (p<0.05) than that stored at 28OC and in egg-yolk citrate extenders at both investigated temperature (p<0.05) 48 hours post extension. Beyond 72 hours of storage, motility in both extenders and at both temperature was less than 40%. The results showed that goat milk in part can replace egg-yolk as a medium for semen extension. Therefore, an extender with concentration of 10-20 % heated goat milk, 70-80% sodium citrate and 10% egg-yolk could be used to store goat semen at 5 OC for 2-3 days.
Keywords: Egg-yolk citrate, Goat-milk citrate, Motility, Semen extension, Spermatozoa
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