Adewuyi et al. 2017

Sokoto Journal of Veterinary Sciences, Volume 15 (Special Issue), 2017

 

 

Evaluation of simplified Folltropin-V® (FSH) protocol on follicular turnover in Yankasa ewes

AB Adewuyi1*, PI Rekwot1, J Rwuaan2, MU Kawu3, M Lawal4, BO Omontese1 & AA Kuje2

1.       Artificial Insemination Unit, National Animal Production Research Institute Ahmadu Bello University, Zaria, Nigeria

2.       Department of Theriogenology and Production Faculty of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria

3.       Department of Veterinary Physiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

4.       Department of Surgery and Radiology Faculty of Veterinary Medicine Ahmadu Bello University, Zaria, Nigeria

 

*Correspondence: Tel.: +2348057373540: E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Abstract

The study was conducted to evaluate the effects of simplifying pFSH (Folltropin-V®) protocol on ovarian follicular turnover in Yankasa ewes. Fifteen Ewes were synchronized for estrus with double injections of 10 mg Dinoprost tromethamine (Lutalyse®) on day 0 and day 10 and randomly allocated to 3 groups. {Cn; control (n= 5, No FSH treatment), F1 (n=5 received FSH once daily) and F2 (n=5, received FSH twice daily at 12 hr. interval)}. Folltropin-V® treatments commenced on Day 8 (equivalent to 80 mg, in decreasing doses over 3 days). Blood samples were collected an hour before and after 1st injection of FSH, then every 12 h over the course of treatment, and then every day till end of estrus. Serum was extracted and assayed for estradiol-17β. Ultrasonic scanning of the ovaries was conducted on day 11. Follicles were counted, measured and classified. Onset of estrus was earlier in F2 than F1 being 16.8 ± 5.0 h and 27.6 ± 4.0 h, respectively. Duration of estrus was shortest for F1 (39.2 ± 11.8 h) and F2 (47.6 ±10.6 h). Estradiol-17β concentrations were elevated in the F1 than F2 1 h after 1st FSH administration, but it was not significant (P > 0.05). Estradiol-17β in F2 (2.7 ± 0.52 pg/ml) was higher than F1 (1.64 ± 0.48 pg/ml) and this was not significant (P > 0.05). A significantly higher number (P < 0.05) of small follicles < 2 mm were observed in F2 (3.6 ± 3.4) than F1 (0.6 ± 0.9). Medium sized follicles 3 mm - 4.5 mm was higher (P > 0.05) in F2 (2.4 ± 2.6) than F1 (0.6 ± 0.9). Number of large follicles >4.5 mm were similar (P > 0.05) being 2.4 ± 2.3 and 1.4 ± 1.2 in F2 and F1 respectively. Both single and double daily FSH protocols were equally efficient in inducing multiple follicular developments.

 

Keywords: Estradiol-17β, Simplified FSH protocol, Superovulation, Ultrasonography, Yankasa ewes

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